An optimized semi-defined medium for p-coumaric acid production in extractive fermentation
نویسندگان
چکیده
In this study, the definition and optimization of a medium for cost-effective heterologous production as well efficient recovery plant secondary metabolite p-coumaric acid (p-CA) was performed. Experimental designs were carried out to end. First, Plackett Burman design used choose important factors that affect p-CA production. Then, central composite face centered find optima two major parameters, C/N ratio pH. Finally, four-component optimized semi-defined (OSD) developed experimented in both biphasic control batch fermentations. comparison results previous fermentations work using classic defined (a 2X Yeast Nitrogen Base without Amino Acids), OSD 10 times less expensive per litre. At pH 4.5, final approximately 61 % content reached medium. 6.0, 55 YNB
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ژورنال
عنوان ژورنال: Process Biochemistry
سال: 2022
ISSN: ['1359-5113', '1873-3298']
DOI: https://doi.org/10.1016/j.procbio.2022.10.021